The Institute of Biomedical Engineering, Department of Cell Biology located at the Medical Faculty of RWTH Aachen University has recently established a new total internal fluorescence reflection/fluorescence recovery after photobleaching (TIRF/FRAP) microscopy system.  The TIRF/FRAP system was generously funded by DFG, NRW government and Medical Faculty of RWTH Aachen University.

 

We provide access to and training on our TIRF/FRAP system as well as advanced live cell imaging and wide-field microscopy (epi-fluorescence, phase contrast, differential interference contrast, multiple fluorophore imaging, etc.).  In addition, we also provide support for cloning and expression of fluorescent proteins (EGFP, RFP, etc.) as well as analysis of TIRF/FRAP images and live cell images.

RFP-Zyxin localisation in Macrophages.
Wide field fluorescence microscopy (pseudo colored in green, left) and TIRF image of RFP-Zyxin (red).

 

FRAP 

 

TIRF

 

For further information please contact:

Dr. Antonio Sechi
Institute of Biomedical Engineering - Department of Cell Biology
Uniklinik RWTH Aachen
Pauwelsstrasse 30, 52074 Aachen, Germany
Phone: +49-241-8085248
FAX: +49-241-8082008
E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.