Welcome to the Institute for Biomedical Engineering, Department of Cell Biology, Medical Faculty of RWTH and Aachen University Hospital, Helmholtz Institute for Biomedical Engineering, Rheinisch Westfälische Technische Hochschule, RWTH, Aachen, Germany.

Cell identity and developmental potential are determined by genetic programs. The laboratory studies genetic programs of hematopoietic stem and progenitor cells, and their differentiated progeny.

Stem cells and progenitor cells are isolated from human cord blood, bone marrow or peripheral blood and from mouse bone marrow, and cells are grown in vitro with specific cytokines. Cells are then induced to differentiate with yet another set of cytokine and/or differentiation factors. A particular focus is on studying mechanisms of genetic and epigenetic regulation, which impact on development of antigen presenting dendritic cells.

 Complete list of publications

 
Stem cell research – Current scientific and societal developments.
Zenke, M., Marx-Stölting and Schickl, H., Editors (2018).
Berlin-Brandenburg Academy of Sciences and Humanities, Nomos Publisher, Baden-Baden, Germany.
 
Differentiation of human induced pluripotent stem cells (iPS cells) and embryonic stem cells (ES cells) into dendritic cell (DC) subsets.
Sontag, S., Förster, M., Seré, K. and Zenke, M. (2017).
Bio-protocol 7, e2419.
 
Modelling IRF8 deficient human hematopoiesis and dendritic cell development with engineered induced pluripotent stem cells.
Sontag, S., Förster, M., Qin, J., Wanek, P., Mitzka, S., Schüler, H. M., Koschmieder, S., Rose-John, S., Seré, K. and Zenke, M. (2017).
Stem Cells 35, 898-908.
 
Surface-grafted nanogel arrays direct cell adhesion and motility.
Sechi, A, Freitas, J, Wünnemann, P, Töpel, A, Paschoalin, RT, Ullmann, S, Schröder, R, Aydin, G, Rütten, S, Böker, A, Zenke, M and Pich, A. (2016).
Adv Mater Int, 1600455, doi: 10.1002/admi.201600455.
 
Analysis of computational footprinting methods for DNase sequencing experiments.
Gusmao, E. G., Allhoff, M., Zenke, M., and Costa, I. G. (2016).
Nature Methods 13, 303-309 (see also Editorial Nature Methods 13, 185)  Abstract Full
 
Epigenetic program and transcription factor circuitry of dendritic cell development.
Lin, Q., Chauvistré, H., Costa, I. G., Gusmão, E. G., Mitzka, S., Haenzelmann, S., Baying, B., Klisch, T., Moriggl, R., Hennuy, B., Smeets, H., Hoffmann, K., Benes, V., Seré, K., and Zenke, M. (2015).
Nucl. Acid Res. 43, 9680-9693.  Abstract Full
 
Crucial role for the LSP1-Myosin 1e bi-molecular complex in the regulation of Fcγ receptor-driven phagocytosis.
Maxeiner, S., Shi, N., Schalla, C., Aydin, G., Hoss, M., Vogel, S., Zenke, M., and Sechi, A. S. (2014).
Mol. Biol. Cell 26, 1652-1664.  Abstract | Full
 
The clash of Langerhans cell homeostasis: Should I stay or should I go?
Hieronymus, T., Zenke, M., Baek, J. H., and Seré, K. (2014).
Semin. Cell. Dev. Biol., S1084-9521. Abstract | Full
 
Cell fusion enhances mesendodermal differentiation of human induced pluripotent stem cells.
Qin, J., Sontag, S., Lin, Q., Mitzka, S., Leisten, I., Schneider, R. K., Wang, X., Jauch, A., Peitz, M., Brüstle, O., Wagner, W., Zhao, R. C., and Zenke, M. (2014).
Stem Cells Dev. 23, 2875-2482. Abstract | Full
 
Dendritic cell development requires histone deacetylase activity.
Chauvistré, H., Küstermann, C., Rehage, N., Klisch, T., Mitzka, S., Felker, P., Rose-John, S., Zenke, M., and Seré, K. (2014).
Eur. J. Immunol. 44, 2478-2488.
 
The polycomb protein Ezh2 impacts on induced pluripotent stem cell generation.
Ding, X., Wang, X., Sontag, S., Qin, J., Wanek, P., Lin, Q., and Zenke, M. (2014).
Stem Cells Dev. 23, 931-940. Abstract | Full
 
Two distinct types of Langerhans cells populate the skin during steady state and inflammation.
Seré, K., Baek, J. H., Ober-Blöbaum, J., Müller-Newen, G., Tacke, F., Yokota, Y., Zenke, M., and Hieronymus, T. (2012).
Immunity 37, 905-916.
 
The HGF receptor/met tyrosine kinase is a key regulator of dendritic cell migration in skin immunity.
Baek, J. H., Birchmeier, C., Zenke, M., and Hieronymus, T. (2012).
J. Immunol. 189, 1699-1707.
 
TGF-ß1 accelerates dendritic cell differentiation from common dendritic cell progenitors and directs subset specification toward conventional dendritic cells.
Felker, P., Seré, K., Lin, Q., Becker, C., Hristov, M., Hieronymus, T., and Zenke, M. (2010).
J. Immunol. 185, 5326-5335.
 
Human adult germline stem cells in question.
Ko, K., Araúzo-Bravo, M. J., Tapia, N., Kim, J., Lin, Q., Bernemann, C., Han, D. W., Gentile, L., Reinhardt, P., Greber, B., Schneider, R. K., Kliesch, S., Zenke, M., and Schöler, H. R. (2010).
Nature 465, E1-E3.  Abstract | Full
 
Pluripotent stem cells induced from adult neural stem cells by reprogramming with two factors.
Kim, J. B., Zaehres, H., Wu, G., Gentile, L., Ko, K., Sebastiano, V., Arauzo-Bravo J. M., Ruau, D., Han, D. W., Zenke, M., and Schöler H. R. (2008).
Nature 54, 646-650.
 
Pluripotency associated genes are reactivated by chromatin modifying agents in neurosphere cells.
Ruau, D., Ensenat-Waser, R., Dinger, T. C., Vallabhapurapu, D. S., Rolletschek, A., Hacker, C., Hieronymus, T., Wobus, A. M., Müller, A. M., and Zenke, M. (2008).
Stem Cells 26, 920-926.
 
Assessment of stem cell/biomaterial combinations for stem cell-based tissue engineering. 
Neuss, S., Apel, C., Buttler, P., Denecke, B., Dhanasingh, A., Ding, X., Grafahrend, D., Groger, A., Hemmrich, K., Herr, A., Jahnen-Dechent, W., Mastitskaya, S., Perez-Bouza, A., Rosewick, S., Salber, J., Wöltje, M., and Zenke, M. (2008).
Biomaterials 29, 302-313.  Abstract | Full
 
Towards an understanding of the transcription factor network of dendritic cell development. 
Zenke, M., and Hieronymus, T. (2006).
Trends Immunol. 27, 140-145.
 
Progressive and controlled development of mouse dendritic cells from Flt3+CD11b+ progenitors in vitro. 
Hieronymus, T., Gust, T. C., Kirsch, R. D., Jorgas, T., Blendinger, G., Goncharenko, M., Supplitt, K., Rose-John, S., Muller, A. M., and Zenke, M. (2005).
J. Immunol. 174, 2552-2562.
 
RNA-containing adenovirus/polyethylenimine transfer complexes effectively transduce dendritic cells and induce antigen-specific T cell responses. 
Gust, T. C., Diebold, S. S., Cotten, M., and Zenke, M. (2004).
J. Gene Med. 6, 464-470.
Abstract | Full
 
Transcriptional profiling identifies Id2 function in dendritic cell development. 
Hacker. C., Kirsch, R. D., Ju, X.-S., Hieronymus, T., Gust, T. C., Kuhl, C., Jorgas, T., Kurz, S. M., Rose-John, S., Yokota, Y., and Zenke, M. (2003).
Nat. Immunol. 4, 380-386.  Abstract | Full
 
The impact of c-met/scatter factor receptor on dendritic cell migration. 
Kurz S. M., Diebold S. S., Hieronymus T., Gust T. C., Bartunek P., Sachs M., Birchmeier W. and Zenke M. (2002).
Eur. J. Immunol. 32, 1832-1838.  Abstract | Full
 
MHC class II presentation of endogenously expressed antigens by transfected dendritic cells. 
Diebold, S. S., Cotten, M., Koch, N., and Zenke, M. (2001).
Gene Ther. 8, 487-493.
 
Mannose polyethylenimine conjugates for targeted DNA delivery into dendritic cells. 
Diebold, S. S., Kursa, M., Wagner, E., Cotten, M., and Zenke, M. (1999).
J. Biol. Chem. 274, 19087-19094.
 
Growth and differentiation of human stem cell factor/erythropoietin-dependent erythroid progenitor cells in vitro.
Panzenböck, B., Bartunek, P., Mapara, M. Y., and Zenke, M. (1998).
Blood 92, 3658-3668.  Abstract | Full
 
Retinoid X receptor and c-cerbA/thyroid hormone receptor regulate erythroid cell growth and differentiation
Bartunek, P., and Zenke, M. (1998).
Mol. Endocrinol. 12, 1269-1279.  Abstract | Full
 
Dendritic cell progenitor is transformed by a conditional v-Rel estrogen receptor fusion protein v-RelER. 
Boehmelt, G., Madruga, J., Dorfler, P., Briegel, K., Schwarz, H., Enrietto, P. J., and Zenke, M. (1995).
Cell 80, 341-352.
 
Ectopic expression of a conditional GATA-2/estrogen receptor chimera arrests erythroid differentiation in a hormone-dependent manner. 
Briegel, K., Lim, K. C., Plank, C., Beug, H., Engel, J. D., and Zenke, M. (1993).
Genes Dev. 7, 1097-1109.  Abstract | Full
 
Receptor-mediated endocytosis of transferrin-polycation conjugates: An efficient way to introduce DNA into hematopoietic cells. 
Zenke, M., Steinlein, P., Wagner, E., Cotten, M., Beug, H., and Birnstiel, M. L. (1990).
Proc. Natl. Acad. Sci. USA 87, 3655-3659.  Abstract | Full
 
v-erbA oncogene activation entails the loss of hormone-dependent regulator activity of c-erbA. 
Zenke, M., Munoz, A., Sap, J., Vennström, B., and Beug, H. (1990).
Cell 61, 1035-1049.  Abstract | Full
 
v-erbA specifically suppresses transcription of the avian erythrocyte anion transporter (band 3) gene. 
Zenke, M., Kahn, P., Disela, C., Vennstrom, B., Leutz, A., Keegan, K., Hayman, M. J., Choi, H. R., Yew, N., Engel, J. D., et al. (1988).
Cell 52, 107-19.  Abstract | Full
 
Multiple sequence motifs are involved in SV40 enhancer function. 
Zenke, M., Grundstrom, T., Matthes, H., Wintzerith, M., Schatz, C., Wildeman, A., and Chambon, P. (1986).
EMBO J. 5, 387-97.  Abstract | Full

  

PubMed Link (to last publications)

Positions available: PhD Student Position (0)   Master Student Position (1)   MTA/BTA Technician (0)

We are always searching for excellent and highly motivated people to join the team for bachelor, master, doctoral or postdoc work. We welcome informal enquiries at any time. Applications should include a cover letter, CV and copies of the most important certificates sent by e-mail.

  

Master Student Position

We seek students with a genuine interest in laboratory work. Interest in working with cellular systems, classical immunohistological techniques and light microscopy analysis of cytoskeletal and signalling proteins is required.  Ideally, before beginning of the master thesis, specific training [8 weeks; valid as research internship (Forschungspraktikum)] on cell culture techniques, biochemistry and microscopy/imaging methods for acquisition of basic experimental knowledge and insight into the laboratory routine should be completed.

We offer individual projects with emphasis on molecular and cellular basis of actin cytoskeleton dynamics, cell adhesion and motility and interaction of cells with biomaterials (1-5).  The official language of our Institute is English.  The application should include cover letter, C.V. and exam certificates.  For application please contact:

Dr. Antonio Sechi

Universitätsklinikum, RWTH Aachen

Institut für Biomedizinische Technologie - Zellbiologie

E-Mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Web pages:

http://www.molcell.rwth-aachen.de/index.php/research/75-regulation-of-actin-cytoskeleton-dynamics

http://www.molcell.rwth-aachen.de/index.php/research/97-tirf-frap-imaging-system

 

References:

1.      Paschoalin, R.T., Traldi, B., Aydin, G., Oliveira, J.E., Rütten, S., Mattoso, L.H.C., Zenke, M. and Sechi, A. (2017).  Solution Blow Spinning Fibres: New Immunologically Inert Substrates for the Analysis of Cell Adhesion and Motility. Acta Biomaterialia, 51: 161-174.

2.      Sechi, A, Freitas, J, Wünnemann, P, Töpel, A, Paschoalin, RT, Ullmann, S, Schröder, R, Aydin, G, Rütten, S, Böker, A, Zenke, M and Pich, A. (2016). Surface-grafted nanogel arrays direct cell adhesion and motility.  Advanced Materials Interfaces, in press.

3.      Gamper I, Fleck D, Barlin M, Spehr M, El Sayad S, Kleine H, Maxeiner S, Schalla C, Aydin G, Hoss M, Litchfield DW, Lüscher B, Zenke M and Sechi, A.* (2016). GAR22b regulates cell migration, sperm motility and axoneme structure. Mol. Biol. Cell. 27: 277-294

4.      Maxeiner, S., Shi, N., Schalla, C., Aydin, G., Hoss, M., Vogel, S., Zenke, M. and Sechi, A.S. (2015). Crucial role for the LSP1-myosin1e bi-molecular complex in the regulation of Fcg receptor-driven phagocytosis. Mol. Biol. Cell. 26: 1652-1664.

5.      Shokouhi, B., Coban, C., Hasirci, V., Aydin, E., Dhanasingh, A., Shi, N., Koyama, S., Akira, S., Zenke, M. and Sechi, A.S. (2010). The role of multiple Toll-like receptor signalling cascades on interactions between biomedical polymers and dendritic cells.

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